Phosphorylation of hepatic phenobarbital-inducible cytochrome P-450.
نویسندگان
چکیده
منابع مشابه
Heterogeneous expression of phenobarbital-inducible cytochrome P-450 genes within the hepatic acinus in the rat.
Within the hepatic acinus, the functional unit of liver parenchyma, the induction of cytochrome P-450 protein by phenobarbital is manifested primarily in hepatocytes located closer to the hepatic venule, i.e., distal hepatocytes. The objective of this study was to determine the levels of cytochromes P-450b and P-450e mRNAs in populations of hepatocytes originating in the proximal or distal half...
متن کاملCharacterization of a phenobarbital - inducible cytochrome P - 450 , NADPH - cytochrome P - 450 reductase and reconstituted cytochrome P - 450 mono - oxygenase system from rat brain
Cytochrome P-450 was purified to apparent homogeneity from the brain microsomes of phenobarbital-treated rats. The specific content of the purified P-450 was 12.7 nmol/mg of protein. NADPH-cytochrome P-450 reductase (reductase) was also purified to apparent homogeneity from brain microsomes. The specific content was 34.7,mol of cytochrome c reduced/min per mg of protein. The reduced carbon mono...
متن کاملMechanism of induction of cytochrome P-450 by phenobarbital.
Treatment of rats with phenobarbital (PB) leads to a substantial increase in the hepatic levels of translatable polysomal poly(A) + cytochrome P-450 mRNA. An enriched fraction of P-450 mRNA was obtained by agarose gel electrophoresis and used to prepare a cDNA probe by differential hybridization to total mRNA from control and PB-treated rats. The majority of the sequences within the probe hybri...
متن کاملNickel carbonyl inhibition of phenobarbital induction of hepatic cytochrome P-450.
Administration of phénobarbital (7.5 mg/100 gm) to rats for 2 days increased the mean concentration of hepatic cytochrome P-450 from 2.1 ±0.5 units to 5.1 ±0.8 units, and increased the mean hepatic weight from 4.4 ±0.3 percent of body weight to 5.0 ± 0.4 percent. Phénobarbital induction of cytochrome P-450 and phénobarbitalstimulation of hepatic enlargement were blocked by injection of n...
متن کاملFractionation and purification of hepatic microsomal cytochrome P-450 isoenzymes from phenobarbital-pretreated rats
A procedure incorporating the salient features of ion-exchange column chromatography with ion-exchange h.p.l.c. is described for the fractionation and purification to homogeneity of several membrane-bound rat hepatic phenobarbital (PB)-inducible cytochrome P-450 isoenzymes, including the major PB-inducible species. The resolving power of this technique makes it a highly promising tool for the i...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: The EMBO Journal
سال: 1989
ISSN: 0261-4189
DOI: 10.1002/j.1460-2075.1989.tb08450.x